413 State Hall, Department of Computer Science, Wayne State University, Detroit MI 48202
Phone: (313) 577-5070 Fax: (313) 577-5070

 

Welcome to Intelligent Systems and Bioinformatics Laboratory
Thank you for using the TAQ software

Upload your data file: (Example File)

Set up the Minimum efficiency for your experiment:

(for example 0.5)

Instructions:

First, please upload your pcr data. Here is an example file. Then set up the minimum efficiency for your experiment. Finally press the submit button. Your result will be reported as a table listed below.

Report For Result:

ID Init_amount Efficiency Residual Starting cycle Interval t-value p-value
mRNA_1 2.638E-9 0.9655 0.03212 27 5 20.6525 0.0034

Annotations:

ID The Name of your mRNA sample
Init_amount The initial amount of molecular in your mRNA sample
Efficiency The efficiency of the polymerase chain reaction., where n is the cycle number, e is the efficiency and En is the product in cycle n.
Residual The mean square error of the log-linear fit
Starting cycle The starting cycle # of log-linear fit in PCR
Interval The interval that fit the log-linear model
t-value t-value of t-test for log-linea fit
p-value p-value of t-test for log linear fit

 

Method Explanation:

Fig. 1. Computational method utilized by the TAQ-software. (a) Real time PCR data. Real time PCR estimates the amount product by measuring DNA specific fluorescent dye intensities after each cycle of amplification. In this figure, the x-axis depicts the cycle number, while the y-axis shows the fluorescence measured. (b) Estimating initial target amount. The TAQ-software transforms the fluorescence data using a natural log and utilizes a linear regression to estimate the initial amount (y-intercept) of fluorescence and reaction efficiency (slope). In the above data, only 4 or 5 points fall within the log-linear portion of the curve.

 

Contact us : Dr. Sorin Draghici - 
              or    Zhandong Liu        - 

 

Webmaster - Valmik Desai -

copyright © 2003 Intelligent Systems and Bioinformatics Laboratory, Computer Science Department, Wayne State University